paiB [2019-07-10 09:53:52]

transcriptional repressor of sporulation and degradative enzyme genes

locus: BSU32140

pI: 5.03

mw: 23.96 kDa

protein length: 207 aa

gene length: 624 bp

function: regulation of sporulation, degradative enzyme and motility genes

product: transcriptional repressor

essential: no

synonyms: yumE

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,304,096 3,304,719

The protein

Protein family

• paiB family (according to Swiss-Prot)

Structure

• [PDB|2OL5] (from Geobacillus stearothermophilus, 39% identity) [pubmed|21633969]

Expression and Regulation

Operons

• genes: [gene|430E15D61FCCA20FAC2BCCF504A12070794372FD|paiA]-[gene|511CFC625D79AAA8B78809651684E4E409337AFA|paiB]
  description: [Pubmed|27965289]

Biological materials

Mutant

• MGNA-A088 (paiB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/88 NBRP B. subtilis, Japan]
• BKE32140 ([gene|511CFC625D79AAA8B78809651684E4E409337AFA|paiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCATCCACCCTTCGA, downstream forward: _UP4_TAAGCTTACTTTGCTGAAGA
• BKK32140 ([gene|511CFC625D79AAA8B78809651684E4E409337AFA|paiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCATCCACCCTTCGA, downstream forward: _UP4_TAAGCTTACTTTGCTGAAGA

References

• 2108124,27965289,21633969